All data presented are derived from two independent experiments. The Content Value Network is a blockchain project and cryptocurrency built off Ethereum, just like most altcoins nowadays. As the name implies, the Content Value Network touts itself as a decentralized content distribution platform. Good content is reviewed, filtered, and distributed content value network cvnt on the platform, while automatically filtering out spam or low-quality work. We then tested different animal sera in the sVNT assays to demonstrate species-independent performance. Results from mice and rabbits immunized with the SARS-CoV-2 RBD protein demonstrate very potent neutralizing activity in the SARS-CoV-2 sVNT (Fig. 3a).
- All other assays, such as enzyme-linked immunosorbent assay (ELISA) and lateral flow assay (LFA) rapid tests, represent the second assay type, which detects total binding antibodies (BAbs) and is unable to differentiate between BAbs and NAbs6,9,10.
- Content Value Network’s current price is $ 0.595, it has increased +0.7% over the past 24 hours.
- D, Inhibition of SARS-CoV-2 RBD–hACE2 interaction by sera from patients with COVID-19.
- Another advantage of sVNT is its ability to detect SARS-CoV-2 antibodies in a species-independent manner.
- There are a few reasons why CVNT isn’t a smart investment right now.
- The test, which has been validated with two cohorts of patients with COVID-19 in two different countries, achieves 99.93% specificity and 95–100% sensitivity, and differentiates antibody responses to several human coronaviruses.
It can also be used to assess vaccine efficacy during preclinical and clinical trials of different vaccine candidates and to monitor neutralizing titers in vaccinees after mass vaccination in human populations. Another advantage of sVNT is its ability to detect SARS-CoV-2 antibodies in a species-independent manner. Sampling serum for antibody detection is also more reliable than other sampling approaches used for molecular detection, as the target tissues can vary from virus to virus25,26,27.
The cVNT and pVNT platforms remain the only platforms for detection of NAbs. However, both require live viruses and cells, highly skilled operators, and days to obtain results. They are thus not suitable for mass production and testing on a commercial scale, even in the most developed nations. Statistical analysis was performed using GraphPad Prism 7 software.
The altcoin market’s popularity has been exploding over the past few months. While that’s good news for most tokens, there are some obscure coins that are hardly getting https://cryptolisting.org/ any traction whatsoever. The Content Value Network Token (CVNT) is one altcoin that isn’t getting any coverage right now, and there are a few reasons why.
Isotype- and species-independent neutralization
To demonstrate specificity, we tested different panels and confirmed that the SARS-CoV-2 sVNT can differentiate antibody responses to SARS-CoV-2 from those to other human CoV infections (Fig. 3c). For human sera from patients with 229/NL63 or OC43 infection and alpaca sera from experimental MERS-CoV infection, there is no detectable cross-neutralization. For SARS sera, there is some level of cross-reactivity, not unexpected given their close genetic relatedness and what was reported previously3,7.
What is the Historical Minimum Price (All-Time Low) of Content Value Network?
Considering the longer term, Content Value Network has seen a price variation of -5.26% in the last 30 days. Extending the timeline further, the 60-day and 90-day periods show price variations of 3.15% and 9.74%, respectively. View the total and circulating supply of Content Value Network, including details on how the supplies are calculated. An overview showing the statistics of Content Value Network, such as the base and quote currency, the rank, and trading volume. The key datasets used in this study are presented in Supplementary Tables 1–3. Additional datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
Extended Data Fig. 1 Comparison of SARS-CoV-2 RBD and S1 sVNT.
In our study, direct binding was demonstrated using different SARS-CoV-2 proteins conjugated with horseradish peroxidase (HRP). There is a dose-dependent specific binding between hACE2 and the RBD or S1, but not the nucleocapsid (N) protein, with the RBD producing the best binding characteristics (Fig. 1c). We then demonstrated that the specific RBD–hACE2 binding can be blocked or neutralized by COVID-19 sera in a dose-dependent manner, but not by sera from healthy controls (Fig. 1d). Using a panel of 20 convalescent sera, we demonstrated that HRP–RBD performed better in detecting NAbs than HRP–S1 (Extended Data Fig. 1).
To validate the performance of the SARS-CoV-2 sVNT, we tested two different cohorts of positive and negative sera. The assay was performed in two different countries by two independent groups to further ensure reliability and reproducibility. A cutoff at 30% inhibition was chosen from testing over 500 negative human sera. For the first cohort, we tested 175 sera from patients with PCR-confirmed COVID-19 in Singapore collected on days 14–33 after symptom onset and 200 healthy control sera, resulting in 100% specificity and sensitivity of 98.9% (Fig. 4d). For the second cohort, we tested 50 sera from patients with PCR-confirmed COVID-19 in Nanjing, China, sampled on days 27–61 after symptom onset and 200 healthy control sera.
The differences between negative control and COVID-19 test sera were analyzed using an unpaired t-test. The differences between paired SARS serum in SARS-CoV-2 sVNT and SARS-CoV sVNT were analyzed using a paired t-test. Correlations between sVNT and cVNT or pVNT were analyzed using Pearson correlation coefficients.
Mainstream crypto exchanges like Coinbase aren’t going to carry CVNT. Your best bet would be to check out dedicated altcoins exchanges like Binance, Bittrex, and Bitmax. Keep in mind that with a market cap of only $8.8 million, there’s very little liquidity on the market. It will be almost impossible to buy or sell large investments in CVNT. Putting all this aside, the fact that CVNT’s popularity has remained unchanged over the past few months, despite the surge in popularity for Bitcoin and other altcoins, is also a warning sign for investors. If CVNT can’t grab any attention from crypto investors, that’s probably a red flag that the project is doomed to obscurity or failure.
Receive your coins
First, most, if not all, testing currently performed at the large scale is for detection of BAbs alone and does not measure true NAbs; second, the presence of NAbs may or may not correlate with protection. In this study, we have developed a serological platform to tackle the first technical challenge. A panel of 60 COVID-19 sera with different levels of SARS-CoV-2 NAbs first determined by sVNT were chosen for a comparative and correlation study between sVNT and two other VNTs, cVNT and pVNT. 4a–c, all three VNT assays had a good overall correlation, with sVNT–cVNT correlating slightly better than sVNT–pVNT or pVNT–cVNT. The sVNT titer was calculated using the half-maximum inhibitory concentration (IC50; Supplementary Table 2).
The Conscious Value Network price page is just one in Crypto.com Price Index that features price history, price ticker, market cap, and live charts for the top cryptocurrencies. Conscious Value Network’s price today is US$0.5964, with a 24-hour trading volume of $26,306. CVNT is +0.00% in the last 24 hours.CVNT has a max supply of 1 B CVNT.
